概述

  • 产品名称
  • 描述
    兔多克隆抗体to SFRS8
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 500 and 550 of human SFRS8 (NP_004583.2)

  • 阳性对照
    • HeLa whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab72044 was affinity purified using an epitope specific to SFRS8 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 105 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 相关性
    SFRS8 is a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Multiple alternatively spliced variants have been identified. Two alternatively spliced variants have been characterized to date.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • 1190005N23Rik antibody
    • 6330437E22Rik antibody
    • AI197402 antibody
    • AW212079 antibody
    • MGC167082 antibody
    • SFRS 8 antibody
    • Sfrs8 protein antibody
    • Splicing factor arginine/serine rich 8 (suppressor of white apricot Drosophila homolog) antibody
    • Splicing factor arginine/serine rich 8 (suppressor of white apricot homolog Drosophila) antibody
    • Splicing factor arginine/serine rich 8 antibody
    • Splicing factor, arginine/serine-rich 8 antibody
    • Suppressor of white apricot protein homolog antibody
    • SWAP antibody
    see all

Anti-SFRS8 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SFRS8 with ab72044 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SFRS8 antibody (ab72044) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 105 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of SFRS8 by Western Blot of Immunprecipitate.
    ab72044 at 1µg/ml staining SFRS8 in HeLa whole cell lysate immunoprecipitated using ab72044 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-SFRS8 antibody (ab72044)参考文献

ab72044 has not yet been referenced specifically in any publications.

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