概述

  • 产品名称Anti-SFRS2B抗体
    参阅全部 SFRS2B 一抗
  • 描述
    兔多克隆抗体to SFRS2B
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (YRESRYGGSH YSSSGYSNSR YSRYHSSRSH SKSGSSTSSR SASTSKSSSA) of Human SFRS2B (NP_115285).

  • 阳性对照
    • 721_B cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Involved in pre-mRNA alternative splicing.
  • 组织特异性Strongly expressed in pancreas, spleen and prostate. Weakly expressed in lung, liver and thymus.
  • 序列相似性Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • arginine/serine-rich 2B antibody
    • Pre mRNA splicing factor SRP46 antibody
    • Pre-mRNA-splicing factor SRP46 antibody
    • Serine/arginine-rich splicing factor 8 antibody
    • SFRS2B antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 46kD antibody
    • Splicing factor arginine serine rich 2B antibody
    • Splicing factor SRp46 antibody
    • SRP46 antibody
    • SRSF8 antibody
    • SRSF8_HUMAN antibody
    see all

Anti-SFRS2B antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SFRS2B with ab83006 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
  • Anti-SFRS2B antibody (ab83006) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 30 kDa,60 kDa,>90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

Anti-SFRS2B antibody (ab83006)参考文献

ab83006 has not yet been referenced specifically in any publications.

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