重组Anti-SFRP4抗体[EPR9389] (ab154167)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9389] to SFRP4
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-SFRP4抗体[EPR9389]
参阅全部 SFRP4 一抗 -
描述
兔单克隆抗体[EPR9389] to SFRP4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human ovary cancer, uterus cancer, MCF7 and 293T lysates; Human colon tissue, Human ovarian carcinoma tissue. ICC/IF: A549 cells, RAW264.7 cells. WB: Human ovary cancer tissue lysate, MCF7, THP-1 cell lysate, Mouse ovary tissue lysate, bEnd.3, HC11, Raw 264.7 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9389 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154167于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. -
组织特异性
Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). -
序列相似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
结构域
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Secreted. Cytoplasmic in ovarian tumor cells. - Information by UniProt
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数据库链接
- Entrez Gene: 6424 Human
- Entrez Gene: 20379 Mouse
- Entrez Gene: 89803 Rat
- Omim: 606570 Human
- SwissProt: Q6FHJ7 Human
- SwissProt: Q9Z1N6 Mouse
- SwissProt: Q9JLS4 Rat
- Unigene: 658169 Human
see all -
别名
- Frizzled protein antibody
- Frizzled protein human endometrium antibody
- FRP 4 antibody
see all
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SFRP4 with ab154167 at 1/200 (6.805 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue).
Confocal image showing positive staining in RAW 264.7 cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling SFRP4 with ab154167 at 1/500 (2.722 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue).
Confocal image showing positive staining in A549 cell line.
Negative control: T-47D.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell culture supernatant at 10 µl
Lane 2 : THP-1 (human monocytic leukemia monocyte) cell culture supernatant at 10 µl
Lane 3 : Blank control
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell) while cell lysate
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) while cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: T-47D.
In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution. -
All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Human ovary cancer tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) while cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) while cell lysate
Lane 4 : Mouse ovary tissue lysate
Lane 5 : bEnd.3 (mouse brain endothelial cell) while cell lysate
Lane 6 : HC11 (mouse mammary gland epithelial cell) while cell lysate
Lane 7 : Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) while cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution.
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All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Human ovary cancer lysate
Lane 2 : Human uterus cancer lysate
Lane 3 : MCF7 lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab154167 被引用在 15 文献中.
- Adekiya TA et al. In Vivo Evaluation of an Antibody-Functionalized Lipoidal Nanosystem for Schistosomiasis Intervention. Pharmaceutics 14:N/A (2022). PubMed: 35893786
- Jiang H et al. lncRNAS56464.1 as a ceRNA promotes the proliferation of fibroblast-like synoviocytes in experimental arthritis via the Wnt signaling pathway and sponges miR-152-3p. Int J Mol Med 47:N/A (2021). PubMed: 33448322
- Leow MKS et al. Paraneoplastic Secretion of Multiple Phosphatonins From a Deep Fibrous Histiocytoma Causing Oncogenic Osteomalacia. J Clin Endocrinol Metab 106:e2299-e2308 (2021). PubMed: 33462615
- Li W et al. MicroRNA-137-mediated lysine demethylase 4A regulates the recovery of spinal cord injury via the SFRP4-Wnt/ß-Catenin axis. Int J Neurosci N/A:1-14 (2021). PubMed: 33499717
- Li N et al. MeCP2 attenuates cardiomyocyte hypoxia/reperfusion-induced injury via regulation of the SFRP4/Wnt/ß-catenin axis. Biomarkers 26:363-370 (2021). PubMed: 33726573