概述

  • 产品名称Anti-SFRP4抗体
    参阅全部 SFRP4 一抗
  • 描述
    兔多克隆抗体to SFRP4
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from within residues 300 - 400 of Human SFRP4.

    (Peptide available as ab32783.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Hela; MCF7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab32784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human SFRP4 peptide (ab32783).
ICC/IF Use at an assay dependent concentration. PubMed: 25036934

靶标

  • 功能Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • 组织特异性Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
  • 序列相似性Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • 结构域The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Secreted. Cytoplasmic in ovarian tumor cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Frizzled protein antibody
    • Frizzled protein human endometrium antibody
    • FRP 4 antibody
    • FRP4 antibody
    • FrpHE antibody
    • human endometrium antibody
    • Secreted frizzled-related protein 4 antibody
    • sFRP-4 antibody
    • Sfrp4 antibody
    • SFRP4_HUMAN antibody
    see all

Anti-SFRP4 antibody 图像

  • All lanes : Anti-SFRP4 antibody (ab32784) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-SFRP4 antibody (ab32784)参考文献

This product has been referenced in:
  • Mori K  et al. Diabetic osteopenia by decreased ß-catenin signaling is partly induced by epigenetic derepression of sFRP-4 gene. PLoS One 9:e102797 (2014). ICC/IF ; Mouse . Read more (PubMed: 25036934) »
  • Ford CE  et al. The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells. PLoS One 8:e54362 (2013). WB . Read more (PubMed: 23326605) »

See all 2 Publications for this product

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