Anti-SFRP1抗体(ab84003)

概述

  • 产品名称Anti-SFRP1抗体
    参阅全部 SFRP1 一抗
  • 描述
    兔多克隆抗体to SFRP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human SFRP1.

    (Peptide available as ab101296.)

  • 阳性对照
    • This antibody gave a positive signal in Human heart tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

靶标

  • 功能Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • 组织特异性Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • 序列相似性Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • 结构域The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all

Anti-SFRP1 antibody 图像

  • Anti-SFRP1 antibody (ab84003) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-SFRP1 antibody (ab84003)参考文献

ab84003 has not yet been referenced specifically in any publications.

Product Wall

I understand that you may be concerned about non-specific binding of the antibody, therefore please find the immunogen sequence below: ********************** With this immunogen information you will be able to do your on BLAST searches in case you do s...

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Thank you for your reply. Based on the sequences you sent, there is a 100% homology between the ground squirrel sequence and the immunogen used for ab84003. Please find the testing discount details below. As this antibody has only been tested on human...

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Thank you for contacting Abcam. Under our Abpromise, we guarantee our antibodies to work as stated on the datasheet, and if it does not works as stated then we would be happy to replace or refund the antibody. In your case, as this antibody has not bee...

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