概述

  • 产品名称Anti-SFPQ抗体
    参阅全部 SFPQ 一抗
  • 描述
    兔多克隆抗体to SFPQ
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    A region within synthetic peptide: KPGGGPGLST PGGHPKPPHR GGGEPRGGRQ HHPPYHQQHH QGPPPGGPGG, corresponding to N terminal amino acids 217-266 of Human SFPQ

  • 阳性对照
    • HepG2 cell lysate and Human skin and intestinal tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:12500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
  • 疾病相关Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
  • 序列相似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰The N-terminus is blocked.
    Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
    Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus matrix. Predominantly in nuclear matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 100 kDa DNA pairing protein antibody
    • 100 kDa DNA-pairing protein antibody
    • 100 kDa subunit antibody
    • DNA binding p52/p100 complex 100 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • hPOMp100 antibody
    • Polypyrimidine tract binding protein associated splicing factor antibody
    • Polypyrimidine tract-binding protein-associated-splicing factor antibody
    • POMP100 antibody
    • PPP1R140 antibody
    • proline- and glutamine-rich antibody
    • Protein phosphatase 1 regulatory subunit 140 antibody
    • PSF antibody
    • PTB associated splicing factor antibody
    • PTB-associated-splicing factor antibody
    • Sfpq antibody
    • SFPQ_HUMAN antibody
    • Splicing factor antibody
    • Splicing factor proline and glutamine rich antibody
    • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
    • Splicing factor proline/glutamine rich antibody
    see all

Anti-SFPQ antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SFPQ with ab50935 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
  • Anti-SFPQ antibody (ab50935) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
  • ab50935 at 4ug/ml staining human SFPQ in epithelial cells of human intestinal villus by Immunohistochemistry, Paraffin embedded tissue.ab50935 at 4ug/ml staining human SFPQ in epithelial cells of human intestinal villus by Immunohistochemistry, Paraffin embedded tissue.
  • ab50935 at 4ug/ml staining human SFPQ in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.ab50935 at 4ug/ml staining human SFPQ in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.

Anti-SFPQ antibody (ab50935)参考文献

ab50935 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 15 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 10 2007

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