Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 816-866 of Human SFMBT1 (NP_057413).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab114863 was affinity purified using an epitope specific to SFMBT1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 98 kDa.
Use at 2-10 µg/mg of lysate.
Expressed in all cell lines and normal tissues tested, including the thymus.
Contains 4 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain.
Information by UniProt
Western blot - Anti-SFMBT1 antibody (ab114863)
All lanes : Anti-SFMBT1 antibody (ab114863) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 98 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-SFMBT1 antibody (ab114863)
Detection of SFMBT1 by Western Blot of Immunprecipitate.
ab114863 at 1µg/ml staining SFMBT1 in HeLa whole cell lysate immunoprecipitated using ab114863 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"