Anti-SFMBT1抗体(ab114863)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SFMBT1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 816-866 of Human SFMBT1 (NP_057413).

  • 阳性对照
    • HeLa, 293T and Jurkat whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab114863 was affinity purified using an epitope specific to SFMBT1 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab114863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 98 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 组织特异性
    Expressed in all cell lines and normal tissues tested, including the thymus.
  • 序列相似性
    Contains 4 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434L243 antibody
    • MGC93349 antibody
    • OTTMUSP00000030378 antibody
    • OTTMUSP00000030379 antibody
    • OTTMUSP00000030381 antibody
    • Renal ubiquitous protein 1 antibody
    • RU1 antibody
    • Scm like with four mbt domains 1 antibody
    • Scm like with four MBT domains protein 1 antibody
    • Scm related gene containing four mbt domains antibody
    • Scm related gene product containing four mbt domains antibody
    • Scm-like with four MBT domains protein 1 antibody
    • SFMBT antibody
    • SFMBT 1 antibody
    • Sfmbt1 antibody
    • SMBT1_HUMAN antibody
    • Smr antibody
    see all

图片

  • All lanes : Anti-SFMBT1 antibody (ab114863) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 98 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-SFMBT1 antibody (ab114863) at 1 µg/ml

    Lane 1 : HeLa cell lysate, IP with alternative SFMBT1 antibody
    Lane 2 : HeLa cell lysate, IP with ab114863 at 6 µg/ml lysate
    Lane 3 : HeLa cell lysate, IP with control IgG

    Lysates/proteins at 200 µg per lane.

    Developed using the ECL technique.

    Exposure time: 30 seconds

文献

ab114863 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Gel Running Conditions
Reduced Denaturing (3-8% gel)
Loading amount
5 µg
Specification
HeLa cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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提交于 Dec 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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