概述

  • 产品名称Anti-SF3B2抗体
    参阅全部 SF3B2 一抗
  • 描述
    小鼠单克隆抗体to SF3B2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length Human SF3B2 protein (AAH07610, 1 a.a. ~ 637 a.a) with a tag of 26 kDa.

  • 阳性对照
    • HeLa nuclear lysate. Recombinant tagged full length human SF3B2 protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab77154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性SF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Pre mRNA splicing factor SF3b 145 kDa subunit antibody
    • SAP 145 antibody
    • SAP145 antibody
    • SF3b1 antibody
    • SF3B145 antibody
    • SF3b150 antibody
    • Spliceosome associated protein 145 antibody
    • Splicing factor 3b subunit 2 145kDa antibody
    • Splicing factor 3b subunit 2 antibody
    see all

Anti-SF3B2 antibody 图像

  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + Recombinant tagged full length human SF3B2 protein at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 98 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + HeLa nuclear lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 98 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

Anti-SF3B2 antibody (ab77154)参考文献

ab77154 has not yet been referenced specifically in any publications.

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