概述

  • 产品名称Anti-SF3A1抗体
    参阅全部 SF3A1 一抗
  • 描述
    兔多克隆抗体to SF3A1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT, corresponding to amino acids 121-170 of Human SF3A1

  • 阳性对照
    • 721 B lymphoblast cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 功能Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Contains 2 SURP motif repeats.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Pre mRNA processing 21 antibody
    • Pre mRNA splicing factor SF3a antibody
    • PRP21 antibody
    • PRPF21 antibody
    • SAP 114 antibody
    • SAP114 antibody
    • sf3a1 antibody
    • SF3A1_HUMAN antibody
    • SF3a120 antibody
    • Spliceosome associated protein 114 antibody
    • Spliceosome-associated protein 114 antibody
    • Splicing factor 3 subunit 1 antibody
    • Splicing factor 3a subunit 1 120kDa antibody
    • Splicing factor 3A subunit 1 antibody
    see all

Anti-SF3A1 antibody 图像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF3A1 with ab69903 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-SF3A1 antibody (ab69903) at 0.25 µg/ml + 721 B lymphoblast cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

Anti-SF3A1 antibody (ab69903)参考文献

ab69903 has not yet been referenced specifically in any publications.

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