概述

  • 产品名称Anti-SETMAR抗体
    参阅全部 SETMAR 一抗
  • 描述
    山羊多克隆抗体to SETMAR
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    RWQKCVDCNGSYFD

    , corresponding to C terminal amino acids 658-671 of Human SETMAR.

  • 阳性对照
    • Daudi lysate.
  • 常规说明Principal Names – SETMAR; SET domain and mariner transposase fusion gene. Official Gene Symbol - SETMAR. GenBank Accession Number – NP_006506. LocusLink ID - 6419 (human). Gene Ontology terms - transposase activity

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab3823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明Western Blot – Approx 70 - 75 kDa band seen in Daudi lysate.
    Recommended for use at 1.0 - 3.0µg/ml.
    Not tested in other applications.

    Optimal working dilutions / concentrations should be determined by the end user.
  • 靶标

    • 功能Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
    • 组织特异性Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
    • 序列相似性In the N-terminal section; belongs to the histone-lysine methyltransferase family.
      In the C-terminal section; belongs to the mariner transposase family.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • 结构域The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Histone lysine N methyltransferase antibody
      • Histone lysine N methyltransferase SETMAR antibody
      • Hsmar 1 antibody
      • Hsmar1 antibody
      • Mariner transposase Hsmar1 antibody
      • Metnase antibody
      • SET domain and mariner transposase fusion antibody
      • SET domain and mariner transposase fusion gene antibody
      • SET domain and mariner transposase fusion gene containing protein antibody
      • SET domain and mariner transposase fusion gene-containing protein antibody
      • Setmar antibody
      • SETMR_HUMAN antibody
      see all

    Anti-SETMAR antibody 图像

    • ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

    Anti-SETMAR antibody (ab3823)参考文献

    This product has been referenced in:
    • Van Duyne R  et al. Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR. Retrovirology 5:40 (2008). WB ; Human . Read more (PubMed: 18498648) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for your patience. The only suggestion that I have at this point (you have done pretty much everything!) is to try blocking with 3% BSA instead. The originator didn't see high background with this antibody and used a more sensitive ...

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    The samples (in 1.5ml microtubes) were simply put into boiling water. So I would expect the temperature to be almost exactly 100 degrees centigrade.

    As stated on the datasheet we used the following conditions to get our result: ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"