Anti-SETD7抗体- ChIP Grade (ab13731)


  • 产品名称Anti-SETD7抗体- ChIP Grade
    参阅全部 SETD7 一抗
  • 描述
    兔多克隆抗体to SETD7 - ChIP Grade
  • 经测试应用适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide (Human).

  • 阳性对照
    • Hela cell lysate.
  • 常规说明ab13731 does not appear to work in IF using mouse or human cell lines.



Our Abpromise guarantee covers the use of ab13731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ChIP: Use at an assay dependent dilution (PMID 17578910).
    WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
    • 组织特异性Widely expressed. Expressed in pancreatic islets.
    • 序列相似性Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
      Contains 3 MORN repeats.
      Contains 1 SET domain.
    • 结构域The SET domain is necessary but not sufficient for histone methyltransferase activity.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ21193 antibody
      • H3 K4 HMTase antibody
      • H3-K4-HMTase SETD7 antibody
      • H4 lysine 4 specific antibody
      • Histone H3 K4 methyltransferase antibody
      • Histone H3 lysine 4 specific methyltransferase antibody
      • Histone H3-K4 methyltransferase SETD7 antibody
      • Histone H4 K4 methyltransferase antibody
      • Histone lysine N methyltransferase antibody
      • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
      • Histone-lysine N-methyltransferase SETD7 antibody
      • KIAA1717 antibody
      • KMT7 antibody
      • Lysine methyltransferase antibody
      • Lysine N-methyltransferase 7 antibody
      • OTTHUMP00000164543 antibody
      • OTTHUMP00000220049 antibody
      • SET 7 antibody
      • SET 7/9 antibody
      • SET 9 antibody
      • SET D7 antibody
      • SET domain containing (lysine methyltransferase) 7 antibody
      • SET domain containing protein 7 antibody
      • SET domain containing protein 8 antibody
      • SET domain-containing protein 7 antibody
      • SET7 antibody
      • SET7/9 antibody
      • SET9 antibody
      • Setd7 antibody
      • SETD7_HUMAN antibody
      see all

    Anti-SETD7 antibody - ChIP Grade 图像

    • Western blot analysis fof Set7 using ab13731 at 1:500 on 20 ugs of HeLa whole cell lysate.

    Anti-SETD7 antibody - ChIP Grade (ab13731)参考文献

    This product has been referenced in:
    • Narayanan A  et al. The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection. Proc Natl Acad Sci U S A 104:10835-40 (2007). ChIP ; Human . Read more (PubMed: 17578910) »

    See 1 Publication for this product

    Product Wall

    Thank you for your kind reminder for ab13731 and for your patience.

    The re-testing has been finished. This antibody (ab13731: Anti-SETD7 antibody - ChIP Grade) was tested on both MCF-7 and HeLa cell lysate. Please see attached results. A sing...

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    Thank you for your prompt answer and for answering to my further questions.

    This is to confirm that we are going to retest the antibody in HeLa cell lysate and I should have the results by the end of this week or early next week.

    I wi...

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    Thank you for your enquiry regardingab13731 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    The other antibody (ab14820) you have tried was onl...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HL60)
    Loading amount 10 µg
    Specification HL60
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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    提交于 Jul 12 2007