Anti-SETD7抗体(ab114145)

概述

  • 产品名称Anti-SETD7抗体
    参阅全部 SETD7 一抗
  • 描述
    兔多克隆抗体to SETD7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human, Recombinant Fragment
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SETD7.

  • 阳性对照
    • This antibody gave a positive signal in KMT7 / SETD7 / SET7/9 Recombinant Protein as well as both HeLa and PC12 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab114145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • 组织特异性Widely expressed. Expressed in pancreatic islets.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • 结构域The SET domain is necessary but not sufficient for histone methyltransferase activity.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Anti-SETD7 antibody 图像

  • All lanes : Anti-SETD7 antibody (ab114145) at 1 µg/ml

    Lane 1 : Recombinant Human SETD7 protein (ab51285)
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

Anti-SETD7 antibody (ab114145)参考文献

ab114145 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"