概述

  • 产品名称
  • 描述
    兔多克隆抗体to SETD3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 247-296 (AVSSVMTRQN QIPTEDGSRV TLALIPLWDM CNHTNGLTPE DSFALAVASA) of Human SETD3 (NP_954574).

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation.
  • 序列相似性
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily.
    Contains 1 SET domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf154 antibody
    • Chromosome 14 open reading frame 154 antibody
    • DKFZp761E1415 antibody
    • FLJ23027 antibody
    • Histone lysine N methyltransferase setd3 antibody
    • Histone-lysine N-methyltransferase setd3 antibody
    • MGC87236 antibody
    • SET domain containing 3 antibody
    • SET domain containing protein 3 antibody
    • SET domain-containing protein 3 antibody
    • SETD 3 antibody
    • Setd3 antibody
    • SETD3_HUMAN antibody
    see all

Anti-SETD3 antibody 图像

  • Anti-SETD3 antibody (ab84734) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-SETD3 antibody (ab84734)参考文献

ab84734 has not yet been referenced specifically in any publications.

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