概述

  • 产品名称Anti-SET抗体
    参阅全部 SET 一抗
  • 描述
    山羊多克隆抗体to SET
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The peptide sequence used (from AAC50460) differs by one residue from the RefSeq entry (NP_003002) and the three other Genbank protein sequences referenced in the Locuslink entry. It is not known whether this difference is due to a sequencing error or polymophism but the antibody is expected to recognize both sequences

性能

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应用

Our Abpromise guarantee covers the use of ab1339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human SET peptide (ab22807).

Approx 20-25kDa and 40kDa bands seen in Human Kidney extracts.

靶标

  • 功能Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • 组织特异性Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • 疾病相关Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • 序列相似性Belongs to the nucleosome assembly protein (NAP) family.
  • 结构域The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • 翻译后修饰Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • 细胞定位Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Anti-SET antibody 图像

  • SET staining (2µg/ml) of Human Kidney extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Anti-SET antibody (ab1339)参考文献

This product has been referenced in:
  • Ruiz PA  et al. 15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity. J Biol Chem 279:36103-11 (2004). Read more (PubMed: 15199053) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. To our knowledge, ab1339 has yet to be tested in IF and ChIP. Thus far, the antibody has only been tested for application in Western blot. If you decide to go ahead and purchase this product, please let us know how you get o...

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SET antibody was made using the N-term epitope according to AAC50460 (U51924). There is a difference of one amino acid in the peptide epitope compared to BAA08139 (D45198). Please let us know should you require further clarification.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"