小鼠单克隆抗体[2965C2a] to SESN2
Recombinant fragment comprising of 50-200 amino acids from the internal region of Human SESN2 (NM_031459).
Recombinant fragment of human SESN2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
Concentration information loading...
Protein G purified
ab76559 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
Use at an assay dependent dilution.
Western blot - Anti-SESN2 antibody [2965C2a] (ab76559)
Anti-SESN2 antibody [2965C2a] (ab76559) at 2 µg/ml + immunising recombinant protein
Predicted band size:
Observed band size:
50 kDa (
why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"