概述

  • 产品名称Anti-SESN1抗体
    参阅全部 SESN1 一抗
  • 描述
    小鼠多克隆抗体to SESN1
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-551 of Human SESN1

  • 阳性对照
    • HeLa cells (IF/ICC) Transfected 293T cell line (WB)

性能

应用

Our Abpromise guarantee covers the use of ab67156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the sestrin family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110002G11Rik antibody
    • AU044290 antibody
    • MGC118148 antibody
    • MGC138241 antibody
    • MGC142129 antibody
    • OTTMUSP00000022799 antibody
    • p53 activated gene 26 antibody
    • p53 regulated PA26 nuclear protein antibody
    • p53 regulated protein PA26 antibody
    • p53-regulated protein PA26 antibody
    • PA26 antibody
    • RP11-787I22.1 antibody
    • sesn1 antibody
    • SESN1_HUMAN antibody
    • SEST1 antibody
    • sestrin 1 antibody
    • Sestrin-1 antibody
    see all

Anti-SESN1 antibody 图像

  • All lanes : Anti-SESN1 antibody (ab67156) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
  • IHC image of ab67156 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67156, at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SESN1 antibody (ab67156)参考文献

ab67156 has not yet been referenced specifically in any publications.

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