Anti-Seryl-tRNA synthetase抗体(ab50146)

概述

  • 产品名称Anti-Seryl-tRNA synthetase抗体
    参阅全部 Seryl-tRNA synthetase 一抗
  • 描述
    兔多克隆抗体to Seryl-tRNA synthetase
  • 特异性This antibody reacts specifically with 60kDa SRS protein.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HEK293 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab50146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/8000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
IP Use at an assay dependent dilution.

靶标

  • 功能Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • 通路Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • 结构域Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C78314 antibody
    • cytoplasmic antibody
    • EC 6.1.1.11 antibody
    • FLJ36399 antibody
    • sarS antibody
    • Sars1 antibody
    • Serine tRNA ligase 1, cytoplasmic antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • serine--tRNA ligase, cytoplasmic antibody
    • SerRS antibody
    • SERS antibody
    • Seryl tRNA Ser/Sec synthetase antibody
    • Seryl tRNA synthetase antibody
    • Seryl tRNA synthetase cytoplasmic antibody
    • Seryl-tRNA Ser/Sec synthetase antibody
    • Seryl-tRNA synthetase antibody
    • seryl-tRNA synthetase, cytoplasmic antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • Strs antibody
    • SYSC_HUMAN antibody
    see all

Anti-Seryl-tRNA synthetase antibody 图像

  • All lanes : Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/1000 dilution

    Lane 1 : Whole cell lysate from rat hippocampal neurons (untreated)
    Lane 2 : Whole cell lysate from rat hippocampal neurons (treated with neurotrophin)
    Lane 3 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1 + neurotrophin)
    Lane 4 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2 + neurotrophin)
    Lane 5 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1)
    Lane 6 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Alkaline Phosphatase-conjugeted goat anti-rabbit IgG at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/10000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-Seryl-tRNA synthetase antibody (ab50146)参考文献

ab50146 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (hippocampal neurons)
Loading amount 40 µg
Specification hippocampal neurons
Treatment Neurotrophic factor
Gel Running Conditions Reduced Denaturing (10% acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Sep 02 2008

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