Anti-Serum Response Factor SRF (phospho S77)抗体(ab79295)

概述

  • 产品名称Anti-Serum Response Factor SRF (phospho S77)抗体
    参阅全部 Serum Response Factor SRF 一抗
  • 描述
    兔多克隆抗体to Serum Response Factor SRF (phospho S77)
  • 特异性ab79295 detects endogenous levels of Serum Response Factor SFR only when phosphorylated at serine 77.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 77 (LYSPGS).

  • 阳性对照
    • IHC: human brain tissue WB: extracts from Jurkat cells, treated with PMA

性能

应用

Our Abpromise guarantee covers the use of ab79295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

靶标

  • 功能SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 序列相似性Contains 1 MADS-box domain.
  • 翻译后修饰Phosphorylated by PRKDC.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF (phospho S77) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab79295, at 1/50 - 1/100 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295) at 1/1000 dilution

    Lane 1 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins)
    Lane 2 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 110 kDa,120 kDa. We are unsure as to the identity of these extra bands.

Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295)参考文献

ab79295 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.75% · Temperature: 4°C
Sample Pig Cell (Pig Nucleus Pulposus Cells)
Specification Pig Nucleus Pulposus Cells
Permeabilization Yes - TritonX
Fixative Paraformaldehyde
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提交于 Sep 26 2013

Thank you for your reply.

Do you have a positive control sample available for testing with this antibody? This antibody will only detect SFR when phosphorylated on S77. This modification may not be present in your fibroblast sample. The posit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"