Anti-Serum Response Factor SRF (phospho S103)抗体(ab53130)

概述

  • 产品名称Anti-Serum Response Factor SRF (phospho S103)抗体
    参阅全部 Serum Response Factor SRF 一抗
  • 描述
    兔多克隆抗体to Serum Response Factor SRF (phospho S103)
  • 特异性ab53130 detects endogenous levels of Serum Response Factor SRF only when phosphorylated at serine 103. In mouse and rat the modification site is serine 99.
  • 经测试应用适用于: WB, IHC-P, ELISA, EMSAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 103 (S-L-SP-E-M).

  • 阳性对照
    • WB: LOVO cell extracts IHC-P: Human lung carcinoma tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab53130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
EMSA Use at an assay dependent concentration.

靶标

  • 功能SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 序列相似性Contains 1 MADS-box domain.
  • 翻译后修饰Phosphorylated by PRKDC.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF (phospho S103) antibody 图像

  • All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

    Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
    Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130)参考文献

This product has been referenced in:
  • Kawashima T  et al. Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. Proc Natl Acad Sci U S A 106:316-21 (2009). EMSA . Read more (PubMed: 19116276) »
  • Xiao Q  et al. Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2. Am J Physiol Cell Physiol 296:C711-23 (2009). Read more (PubMed: 19036941) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Unfortunately, this specific lysate (LOVO cell extract treated with serum (20%, 15min)) is not available for sale. Alternative positive controls would be a human lung carcinoma lysate (e.g. ab14870 or ab14871...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"