Anti-Serum Response Factor SRF (phospho S103)抗体(ab53130)

概述

  • 产品名称
    Anti-Serum Response Factor SRF (phospho S103)抗体
    参阅全部 Serum Response Factor SRF 一抗
  • 描述
    兔多克隆抗体to Serum Response Factor SRF (phospho S103)
  • 特异性
    ab53130 detects endogenous levels of Serum Response Factor SRF only when phosphorylated at serine 103. In mouse and rat the modification site is serine 99.
  • 经测试应用
    适用于: WB, IHC-P, ELISA, EMSAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 103 (S-L-SP-E-M).

  • 阳性对照
    • WB: LOVO cell extracts IHC-P: Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab53130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
EMSA Use at an assay dependent concentration.

靶标

  • 功能
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 序列相似性
    Contains 1 MADS-box domain.
  • 翻译后修饰
    Phosphorylated by PRKDC.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

图片

  • All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

    Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
    Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

文献

This product has been referenced in:
  • Heller EA  et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). Read more (PubMed: 25347353) »
  • Vissing K  et al. Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway. J Physiol 591:3749-63 (2013). Read more (PubMed: 23753523) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for contacting us. Unfortunately, this specific lysate (LOVO cell extract treated with serum (20%, 15min)) is not available for sale. Alternative positive controls would be a human lung carcinoma lysate (e.g. ab14870 or ab14871...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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