概述
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产品名称Anti-Serum Response Factor SRF抗体
参阅全部 Serum Response Factor SRF 一抗 -
描述兔多克隆抗体to Serum Response Factor SRF
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宿主Rabbit
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特异性This antibody detects endogenous levels of total Serum Response Factor SRF protein.
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经测试应用适用于: ELISA, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse
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免疫原
Synthesized non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of threonine 159 (R-Y-TP-T-F)
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阳性对照
- Extracts from HeLa cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab79378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/20000. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
靶标
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功能SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
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序列相似性Contains 1 MADS-box domain.
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翻译后修饰Phosphorylated by PRKDC.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 6722 Human
- Entrez Gene: 20807 Mouse
- Omim: 600589 Human
- SwissProt: P11831 Human
- SwissProt: Q9JM73 Mouse
- Unigene: 520140 Human
- Unigene: 45044 Mouse
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别名
- c fos serum response element binding factor antibody
- c fos serum response element binding transcription factor antibody
- ELK3 antibody
see all
图片
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Western blot - Serum Response Factor SRF antibody (ab79378)All lanes : Anti-Serum Response Factor SRF antibody (ab79378) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with immunizing peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
文献
ab79378 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"