RabMAb

Anti-Serum Amyloid P抗体[EP1018Y] (ab45151)

概述

  • 产品名称
    Anti-Serum Amyloid P抗体[EP1018Y]
    参阅全部 Serum Amyloid P 一抗
  • 描述
    兔单克隆抗体[EP1018Y] to Serum Amyloid P
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Serum Amyloid P aa 1 to the C-terminus (N terminal).

  • 阳性对照
    • Human kidney and liver tissue. Human Serum.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab45151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Unpurified format: 1/1000000

IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results

靶标

  • 功能
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • 组织特异性
    Found in serum and urine.
  • 疾病相关
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • 序列相似性
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 翻译后修饰
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Anti-Serum Amyloid P antibody [EP1018Y] 图像

  • Anti-Serum Amyloid P antibody [EP1018Y] (ab45151) at 1/10000 dilution + Human serum at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 25 kDa

    Diluting and blocking buffer: 5% NFDM /TBST 

  • ab45151 staining Serum Amyloid P in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Anti-Serum Amyloid P antibody [EP1018Y] (ab45151) at 1/5000 dilution + Human liver at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 25 kDa

    Blocking and diluting buffer: 5% NFDM /TBST

  • Ab45151 (1:100) staining human Amyloid P component in human kidney by immunohistochemistry using paraffin embedded tissue.

Anti-Serum Amyloid P antibody [EP1018Y] (ab45151)参考文献

This product has been referenced in:
  • Helmfors L  et al. Serum Amyloid P Component Ameliorates Neurological Damage Caused by Expressing a Lysozyme Variant in the Central Nervous System of Drosophila melanogaster. PLoS One 11:e0159294 (2016). IHC-P . Read more (PubMed: 27428539) »
  • McCook JP  et al. Ability of sodium copper chlorophyllin complex to repair photoaged skin by stimulation of biomarkers in human extracellular matrix. Clin Cosmet Investig Dermatol 9:167-74 (2016). IHC-P ; Human . Read more (PubMed: 27524916) »

See all 7 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 20 2015

Application
Western blot
Sample
Human Serum (Serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 25 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 22 2014

Thank you for waiting.

I have contacted my lab colleagues who confirmed product ab45151 from lot number 1351175 has a concentration of 0.426 mg/ml.

I hope this helps and if we can assist further, please do not hesitate to contact us.

Thank you for contacting Abcam.

Please find attached the IHC-P protocol that we discussed on the telephone. Also let me know if the protocol variations that we discussed are not successful.

Hope you have a great weekend.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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