Anti-Serum Amyloid P抗体[6E6] (ab51085)


  • 产品名称Anti-Serum Amyloid P抗体[6E6]
    参阅全部 Serum Amyloid P 一抗
  • 描述
    小鼠单克隆抗体[6E6] to Serum Amyloid P
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein purified from Human plasma

  • 阳性对照
    • Human plasma



Our Abpromise guarantee covers the use of ab51085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.


  • 功能Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • 组织特异性Found in serum and urine.
  • 疾病相关Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • 序列相似性Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 翻译后修饰N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Anti-Serum Amyloid P antibody [6E6] 图像

  • All lanes : Anti-Serum Amyloid P antibody [6E6] (ab51085) at 1/1000 dilution

    Lane 1 : Amyloid P protein isolated from human plasma
    Lane 2 : Human plasma

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-Serum Amyloid P antibody [6E6] (ab51085)参考文献

This product has been referenced in:
  • Gu BJ  et al. P2X7 receptor-mediated scavenger activity of mononuclear phagocytes toward non-opsonized particles and apoptotic cells is inhibited by serum glycoproteins but remains active in cerebrospinal fluid. J Biol Chem 287:17318-30 (2012). WB ; Human . Read more (PubMed: 22461619) »

See 1 Publication for this product

Product Wall

Thank you for the clarification about the blocking buffer. BSA should be fine, but if you are seeing no signal at all, you could try reducing the percentage of BSA 10-fold, to 0.5%. This might allow a signal to develop more easily.

Human ser...

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Your protocol looks mostly correct but my one concern is the antibody diluent, 5% FBS. Are you confident the Fetal Bovine Serum does not contain the target of the antibody, serum amyloid P? I think it is likely that the antibody is reacting with this i...

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