Anti-Serum Amyloid P抗体[14B4] (ab27313)

概述

  • 产品名称Anti-Serum Amyloid P抗体[14B4]
    参阅全部 Serum Amyloid P 一抗
  • 描述
    小鼠单克隆抗体[14B4] to Serum Amyloid P
  • 特异性None detected so far.
  • 经测试应用适用于: Sandwich ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native serum Amyloid P protein (purified) from Human plasma.

  • 常规说明


    This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4.
  • Concentration information loading...
  • 纯度Protein A purified
  • Primary antibody说明This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.
  • 克隆单克隆
  • 克隆编号14B4
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab27313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA 1/4000. Can be paired for Sandwich ELISA with Rabbit polyclonal to Serum Amyloid P (ab17872). Use as Capture antibody with recommended pair. See Abreview submitted by Jennifer Webster for details.
WB 1/400. Use under non reducing condition. Predicted molecular weight: 25 kDa.
ELISA 1/4000. Use at an assay dependent dilution.

靶标

  • 功能Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • 组织特异性Found in serum and urine.
  • 疾病相关Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • 序列相似性Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 翻译后修饰N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Anti-Serum Amyloid P antibody [14B4] (ab27313)参考文献

This product has been referenced in:
  • Chen HM  et al. Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach. Clin Biochem 44:377-85 (2011). WB . Read more (PubMed: 21223953) »
  • Li C  et al. Pancreatic cancer serum detection using a lectin/glyco-antibody array method. J Proteome Res 8:483-92 (2009). Read more (PubMed: 19072160) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Purified protein)
Loading amount 0.1 µg
Specification Purified protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

提交于 Mar 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (Serum)
Specification Serum
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type Sandwich
Username

Miss. Jennifer Webster

Verified customer

提交于 Aug 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"