This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1).
Mouse, Rat, Dog
, corresponding to amino acids 186/199 of Human SERPINI2
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
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Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 3 µg/ml.
||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Expressed in pancreas and adipose tissues.
Belongs to the serpin family.
Information by UniProt
- MEPI antibody
- Myoepithelium derived serine protease inhibitor antibody
- Myoepithelium-derived serine protease inhibitor antibody
Western blot - Anti-SERPINI2 antibody (ab36169)
Anti-SERPINI2 antibody (ab36169) at 0.3 µg/ml + Human Pancreas lysate in RIPA buffer for 1 hour. at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Western blot showing MEPI in human pancreas lysate (35 micrograms protein in RIPA buffer). 1 hour primary incubation. Detected by chemiluminescence.(0.3µg/ml)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINI2 antibody (ab36169)
ab36169 (3µg/ml) staining Human Pancreas by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.
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