概述

  • 产品名称Anti-SerpinB3抗体
    参阅全部 SerpinB3 一抗
  • 描述
    小鼠单克隆抗体to SerpinB3
  • 经测试应用适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 276-391 of Human SerpinB3

性能

应用

Our Abpromise guarantee covers the use of ab55733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23185467
IHC-P Use at an assay dependent concentration. PubMed: 23185467

靶标

  • 功能May act as a protease inhibitor to modulate the host immune response against tumor cells.
  • 组织特异性Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
  • 序列相似性Belongs to the serpin family. Ov-serpin subfamily.
  • 发展阶段Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.
  • 细胞定位Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HsT1196 antibody
    • Protein T4 A antibody
    • Protein T4-A antibody
    • SCC antibody
    • SCCA antibody
    • SCCA PD antibody
    • SCCA-1 antibody
    • SCCA-PD antibody
    • SCCA1 antibody
    • SCCAPD antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3 antibody
    • Serpin B3 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 3 antibody
    • serpin peptidase inhibitor, clade B (ovalbumin), member 3 antibody
    • SERPINB3 antibody
    • SPB3_HUMAN antibody
    • Squamous cell carcinoma antigen 1 antibody
    • T4 A antibody
    • T4-A antibody
    • T4A antibody
    see all

Anti-SerpinB3 antibody 图像

  • Overlay histogram showing A431 cells stained with ab55733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55733, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot against tagged recombinant protein immunogen using ab55733 SerpinB3 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa

Anti-SerpinB3 antibody (ab55733)参考文献

This product has been referenced in:
  • Lim W  et al. SERPINB3 in the Chicken Model of Ovarian Cancer: A Prognostic Factor for Platinum Resistance and Survival in Patients with Epithelial Ovarian Cancer. PLoS One 7:e49869 (2012). ICC/IF, IHC-P ; Human, Chicken . Read more (PubMed: 23185467) »
  • Burgener A  et al. Identification of differentially expressed proteins in the cervical mucosa of HIV-1-resistant sex workers. J Proteome Res 7:4446-54 (2008). WB ; Human . Read more (PubMed: 18707157) »

See all 2 Publications for this product

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I can confirm that although we have not tested these antibodies for cross-reactivity with Serpin B4 but there is high percentage of immunogen sequence homology. It means the chances of cross reactivity are high. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"