概述

  • 产品名称Anti-SERPINB1抗体
    参阅全部 SERPINB1 一抗
  • 描述
    兔多克隆抗体to SERPINB1
  • 特异性This antibody is specific for SERPINB1.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide based on the aminoterminal end of mature human SERPINB1

性能

应用

Our Abpromise guarantee covers the use of ab47731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
  • 序列相似性Belongs to the serpin family. Ov-serpin subfamily.
  • 结构域Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anti elastase antibody
    • EI antibody
    • ELANH2 antibody
    • Epididymis luminal protein 57 antibody
    • HEL57 antibody
    • ILEU_HUMAN antibody
    • LEI antibody
    • Leukocyte elastase inhibitor antibody
    • M/NEI antibody
    • MNEI antibody
    • Monocyte/neutrophil elastase inhibitor antibody
    • Peptidase inhibitor 2 antibody
    • Pi-2 antibody
    • PI2 antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 antibody
    • Serpin B1 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 1 antibody
    • serpinb1 antibody
    see all

Anti-SERPINB1 antibody 图像

  • All lanes : Anti-SERPINB1 antibody (ab47731) at 1/1000 dilution

    Lane 1 : MH-S cell lysate
    Lane 2 : MH-S cell lysate
    Lane 3 : MH-S cell lysate

    Lysates/proteins at 20 µl per lane.


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa (possible degradation product).
  • ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-SERPINB1 antibody (ab47731)参考文献

This product has been referenced in:
  • Sommeregger W  et al. Proteomic differences in recombinant CHO cells producing two similar antibody fragments. Biotechnol Bioeng 113:1902-12 (2016). WB . Read more (PubMed: 26913574) »
  • Uchiyama K  et al. Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis. Am J Physiol Gastrointest Liver Physiol 302:G1163-70 (2012). WB, IHC-P ; Human . Read more (PubMed: 22421620) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"