Highly pure (>98%) recombinant full length human SERPINA12.
Lyophilised:Reconstitute in 500 µl sterile PBS containing 0.1% BSA to a concentration of 0.1 mg/ml.
SDS states: Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. [Checker:I have entered 0.1 mg/ml on Details page for porotein conc.]
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: (Direct or Sandwich) Use at a concentration of 0.25 - 1 µg/ml, at 100µl per well.
This allows the detection of at least 0.2 - 0.4 ng/well of recombinant SERPINA12.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 46 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant SERPINA12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.