Anti-AANAT抗体(ab3505)
Key features and details
- Rabbit polyclonal to AANAT
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
概述
-
产品名称
Anti-AANAT抗体
参阅全部 AANAT 一抗 -
描述
兔多克隆抗体to AANAT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse AANAT aa 1-100 (N terminal).
Database link: O88816 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide. -
Primary antibody说明
Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. |
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. |
靶标
-
功能
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. -
组织特异性
Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland. -
通路
Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. -
疾病相关
Delayed sleep phase syndrome -
序列相似性
Belongs to the acetyltransferase family. AANAT subfamily.
Contains 1 N-acetyltransferase domain. -
翻译后修饰
cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 503504 Chimpanzee
- Entrez Gene: 15 Human
- Entrez Gene: 11298 Mouse
- Entrez Gene: 25120 Rat
- Omim: 600950 Human
- SwissProt: P79774 Chicken
- SwissProt: Q5IS55 Chimpanzee
- SwissProt: Q16613 Human
see all -
别名
- AA-NAT antibody
- Aanat antibody
- Aralkylamine N-acetyltransferase antibody
see all
数据表及文件
-
Datasheet download
文献 (12)
ab3505 被引用在 12 文献中.
- Yang C et al. Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserve. Commun Biol 4:534 (2021). PubMed: 33958705
- Yang M et al. Melatonin promotes male reproductive performance and increases testosterone synthesis in mammalian Leydig cells†. Biol Reprod 104:1322-1336 (2021). PubMed: 33709108
- Wang J et al. Melatonin Alleviates the Suppressive Effect of Hypoxanthine on Oocyte Nuclear Maturation and Restores Meiosis via the Melatonin Receptor 1 (MT1)-Mediated Pathway. Front Cell Dev Biol 9:648148 (2021). PubMed: 33937242
- Song L et al. Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway. Nutrients 14:N/A (2021). PubMed: 35010992
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343