RabMAb

Anti-Serine/threonine-protein kinase 4抗体[EP1465Y] (ab51134)

概述

  • 产品名称Anti-Serine/threonine-protein kinase 4抗体[EP1465Y]
    参阅全部 Serine/threonine-protein kinase 4 一抗
  • 描述
    兔单克隆抗体[EP1465Y] to Serine/threonine-protein kinase 4
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Serine/threonine-protein kinase 4 aa 1-100 (N terminal).

  • 阳性对照
    • WB: Jurkat and HeLa cell lysate and mouse spleen, rat spleen and human urinary bladder tissues. ICC/IF: Raw264.7 cells. IHC-P:Human gastric carcinoma. IP: Jurkat cell lysate. Flow Cyt: HeLa cells.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50.
WB 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/250.

We strongly recommend that customers perform an antigen retrieval step.

IHC-Fr Use at an assay dependent concentration. PubMed: 24595170
ICC/IF 1/100 - 1/250.
IP 1/30 - 1/100.

靶标

  • 功能Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • 翻译后修饰Autophosphorylated on serine and threonine residues.
  • 细胞定位Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Kinase responsive to stress antibody
    • Krs2 antibody
    • Mammalian STE20 like protein kinase 1 antibody
    • Mammalian STE20-like protein kinase 1 antibody
    • Mammalian sterile 20 like 1 antibody
    • MST-1 antibody
    • MST1 antibody
    • Serine/threonine kinase 4 antibody
    • Serine/threonine protein kinase Krs 2 antibody
    • Serine/threonine-protein kinase 4 antibody
    • Serine/threonine-protein kinase Krs-2 antibody
    • STE20 like kinase MST1 antibody
    • STE20-like kinase MST1 antibody
    • STK4 antibody
    • STK4_HUMAN antibody
    • TIIAC antibody
    • YSK3 antibody
    see all

Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] 图像

  • ab51134 staining Serine/threonine-protein kinase 4 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000)  were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • ab51134 staining Serine/threonine-protein kinase 4 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] (ab51134) at 1/50000 dilution

    Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 2 : Mouse spleen
    Lane 3 : Rat spleen

    Lysates/proteins at 1/20 dilution per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 56 kDa

    Diluting and blocking buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab51134 immunoprecipitating Serine/threonine-protein kinase 4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate

  • Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] (ab51134) at 1/10000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 56 kDa

    Diluting and blocking buffer: 5% NFDM/TBST

Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] (ab51134)参考文献

This product has been referenced in:
  • Wang T  et al. Mst1 participates in the atherosclerosis progression through macrophage autophagy inhibition and macrophage apoptosis enhancement. J Mol Cell Cardiol 98:108-116 (2016). WB ; Mouse . Read more (PubMed: 27496379) »
  • Hu J  et al. OSM mitigates post-infarction cardiac remodeling and dysfunction by up-regulating autophagy through Mst1 suppression. Biochim Biophys Acta N/A:N/A (2016). WB ; Mouse . Read more (PubMed: 27825852) »

See all 4 Publications for this product

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