概述

  • 产品名称
    Anti-SERCA3 ATPase抗体
    参阅全部 SERCA3 ATPase 一抗
  • 描述
    兔多克隆抗体to SERCA3 ATPase
  • 宿主
    Rabbit
  • 特异性
    Detects sarcoplasmic or endoplasmic reticulum calcium ATPase 3 (SERC A3) human and rat recombinant protein. This antibody does not detect other SERCA isoforms.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse SERCA3 ATPase aa 29-39.
    Sequence:

    VTDARERYGPN


    (Peptide available as ab4955)

性能

应用

Our Abpromise guarantee covers the use of ab3339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 113 kDa).Can be blocked with SERCA3 ATPase peptide (ab4955). By Western blot, this antibody detects an ~97 kDa protein representing recombinant SERCA3 from rat samples.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • 组织特异性
    Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
  • 序列相似性
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • 细胞定位
    Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenosine triphosphatase calcium antibody
    • AT2A3_HUMAN antibody
    • Atp2a3 antibody
    • ATPase Ca(2+) transporting ubiquitous antibody
    • ATPase Ca++ transporting ubiquitous antibody
    • Calcium pump 3 antibody
    • Calcium translocating P type ATPase antibody
    • Sarco/endoplasmic reticulum Ca2+ ATPase antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibody
    • SERCA 3 antibody
    • SERCA3 antibody
    • SERCA3b antibody
    • SR Ca(2+) ATPase 3 antibody
    • SR Ca(2+)-ATPase 3 antibody
    see all

图片

  • Western blot of recombinant human SERCA3 ATPase expressed in E. coli using ab3339. Western blot of recombinant human SERCA3 ATPase expressed in E. coli using ab3339.

文献

This product has been referenced in:
  • Poch E  et al. Functional characterization of alternatively spliced human SERCA3 transcripts. Am J Physiol 275:C1449-58 (1998). Read more (PubMed: 9843705) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Loading amount
30 µg
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册