Anti-SERCA1 ATPase抗体(ab92666)

概述

  • 产品名称Anti-SERCA1 ATPase抗体
    参阅全部 SERCA1 ATPase 一抗
  • 描述
    兔多克隆抗体to SERCA1 ATPase
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized peptide corresponding to an internal sequence of Human SERCA1 ATPase.

  • 阳性对照
    • NIH 3T3, A549 or HT29 cell lysate. IF/ICC: HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab92666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明ELISA: 1/10,000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
    • 组织特异性Skeletal muscle, fast twitch muscle (type II) fibers.
    • 疾病相关Brody myopathy
    • 序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • 发展阶段Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
    • 细胞定位Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    Anti-SERCA1 ATPase antibody 图像

    • ICC/IF image of ab92666 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92666, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-SERCA1 ATPase antibody (ab92666) at 1/500 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HT29 cell lysate
      Lane 4 : A549 cell lysate with immunizing peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 110 kDa

    Anti-SERCA1 ATPase antibody (ab92666)参考文献

    ab92666 has not yet been referenced specifically in any publications.

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