Anti-Septin 3抗体(ab57478)

概述

  • 产品名称Anti-Septin 3抗体
    参阅全部 Septin 3 一抗
  • 描述
    小鼠单克隆抗体to Septin 3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 236-346 of Human Septin 3

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
    • 组织特异性Brain-specific.
    • 序列相似性Belongs to the septin family.
    • 翻译后修饰Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bK250D10.3 antibody
      • MGC133218 antibody
      • Neuronal specific septin 3 antibody
      • Neuronal-specific septin-3 antibody
      • OTTHUMP00000028742 antibody
      • SEP 3 antibody
      • SEP3 antibody
      • SEPT 3 antibody
      • sept3 antibody
      • SEPT3 protein antibody
      • SEPT3_HUMAN antibody
      • Septin3 antibody
      see all

    Anti-Septin 3 antibody 图像



    • Predicted band size : 40 kDa
      Septin 3 antibody (ab57478) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
    • Anti-Septin 3 antibody (ab57478) at 3 µg/ml + Lysates prepared from human Vero cells at 20000 cells

      Secondary
      HRP conjugated sheep polyclonal to mouse IgG at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 40 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      This image is a courtesy of Anonymous Abreview

      See Abreview

    Anti-Septin 3 antibody (ab57478)参考文献

    ab57478 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    提交于 Jun 23 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"