概述

  • 产品名称Anti-Septin 11抗体
    参阅全部 Septin 11 一抗
  • 描述
    兔多克隆抗体to Septin 11
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide within residues: AVAVGRPSNE ELRNLSLSGH VGFDSLPDQL VNKSTSQGFC FNILCVGETG, corresponding to N terminal amino acids 1-50 of Human Septin 11 (NP_060713).

  • 阳性对照
    • MCF7 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab86268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.
  • 组织特异性Widely expressed, except in leukocytes.
  • 疾病相关Note=A chromosomal aberration involving SEPT11 may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with MLL/HRX.
  • 序列相似性Belongs to the septin family.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction > synapse. Cell projection > dendritic spine. Cell projection > axon. Partly colocalizes with stress fibers and microtubules. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SEP11_HUMAN antibody
    • SEPT 11 antibody
    • Sept11 antibody
    • Septin-11 antibody
    • Septin11 antibody
    see all

Anti-Septin 11 antibody 图像

  • Anti-Septin 11 antibody (ab86268) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Septin 11 with ab86267 at 5.0µg/ml.

  • All lanes : Anti-Septin 11 antibody (ab86268) at 2 µg/ml

    Lane 1 : Human NT2 cells at 60 µg
    Lane 2 : Mouse brain extracts at 80 µg

    Secondary
    Goat anti-rabbit at 1/2000 dilution

    Predicted band size : 49 kDa
    WB Suggested Anti-Rab11a antibody (ab125456) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain

Anti-Septin 11 antibody (ab86268)参考文献

ab86268 has not yet been referenced specifically in any publications.

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