Anti-SEPT7抗体(ab79286)

概述

  • 产品名称Anti-SEPT7抗体
    参阅全部 SEPT7 一抗
  • 描述
    山羊多克隆抗体to SEPT7
  • 特异性ab79286 is expected to recognize both reported isoforms (NP_001779.3; NP_001011553.2).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide:

    C-DNNKNKGQLTKSP

    , corresponding to internal sequence amino acids 323-335 of Human SEPT7 (NP_001779.3)

  • 阳性对照
    • HeLa nuclear cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab79286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab79286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-SEPT7 antibody 图像

    Anti-SEPT7 antibody (ab79286)参考文献

    ab79286 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Peritoneal macrophages)
    Permeabilization Yes - 0,25% Triton
    Specification Peritoneal macrophages
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative Formaldehyde
    Username

    Dr. Manoj B Menon

    Verified customer

    提交于 Apr 24 2015

    Abreviews
    Application Western blot
    Loading amount 10000 cells
    Gel Running Conditions Reduced Denaturing (7.5 to 16% gradient)
    Sample Mouse Cell lysate - whole cell (Bone Marrow Derived Macrophages)
    Specification Bone Marrow Derived Macrophages
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Manoj B Menon

    Verified customer

    提交于 Apr 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"