概述
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产品名称Anti-SEPT7抗体
参阅全部 SEPT7 一抗 -
描述山羊多克隆抗体to SEPT7
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宿主Goat
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特异性ab79286 is expected to recognize both reported isoforms (NP_001779.3; NP_001011553.2).
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Orangutan
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免疫原
Synthetic peptide:
C-DNNKNKGQLTKSP
, corresponding to internal sequence amino acids 323-335 of Human SEPT7 (NP_001779.3) -
阳性对照
- HeLa nuclear cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab79286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab79286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
Peptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements.
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组织特异性Widely expressed.
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序列相似性Belongs to the septin family.
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细胞定位Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers.
- Information by UniProt
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数据库链接
- Entrez Gene: 463349 Chimpanzee
- Entrez Gene: 408000 Cow
- Entrez Gene: 100070492 Horse
- Entrez Gene: 989 Human
- Entrez Gene: 235072 Mouse
- Entrez Gene: 100173884 Orangutan
- Entrez Gene: 64551 Rat
- Omim: 603151 Human
see all -
别名
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
see all
图片
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Western blot - SEPT7 antibody (ab79286)Anti-SEPT7 antibody (ab79286) at 0.5 µg/ml + HeLa nuclear lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
文献
ab79286 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"