概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SEPT6
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    DEVNAFKQRKTA

    by a Cysteine residue linker, corresponding to C terminal amino acids 391-402 of Human SEPT6 (NP_665798.1; NP_055944.2; NP_665801.1).

  • 阳性对照
    • Human testis, thyroid gland and MOLT4 cell lysates
  • 常规说明


    ab106924 is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明
    ab106924 is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 50 kDa.

靶标

  • 功能
    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the septin family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0128 antibody
    • Nkrf antibody
    • RCG53214, isoform CRA_d antibody
    • RP5-876A24.2 antibody
    • SEP2 antibody
    • SEPT2 antibody
    • SEPT6 antibody
    • SEPT6/MLL FUSION GENE antibody
    • SEPT6_HUMAN antibody
    • Septin 2 antibody
    • Septin 6 antibody
    • Septin-6 antibody
    see all

图片

  • ab106944 (3.8µg/ml) staining of paraffin embedded Human Tonsil following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows specific staining in dividing cells just outside a germinal centre.

  • Anti-SEPT6 antibody (ab106944) at 1 µg/ml + Human testis lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa

文献

ab106944 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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