Anti-SEPT4抗体[ARTS51] (ab10440)

概述

  • 产品名称Anti-SEPT4抗体[ARTS51]
    参阅全部 SEPT4 一抗
  • 描述
    小鼠单克隆抗体[ARTS51] to SEPT4
  • 经测试应用适用于: ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human SEPT4 aa 247-266 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    NIQDNRVHCCLYFI SPFGHG

  • 阳性对照
    • Whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS.

性能

应用

Our Abpromise guarantee covers the use of ab10440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 34 kDa.
ICC Use at an assay dependent dilution.

靶标

  • 功能Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
  • 组织特异性Widely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
  • 序列相似性Belongs to the septin family.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis-related protein in the TGF-beta signaling pathway antibody
    • ARTS antibody
    • Bradeion antibody
    • Bradeion beta antibody
    • Brain protein H5 antibody
    • CE5B3 antibody
    • CE5B3 beta antibody
    • Cell division control-related protein 2 antibody
    • Cerebral protein 7 antibody
    • H5 antibody
    • hCDCREL-2 antibody
    • hucep-7 antibody
    • MART antibody
    • Peanut-like protein 2 antibody
    • PNUTL2 antibody
    • SEP 4 antibody
    • SEP4 antibody
    • Sept4 antibody
    • SEPT4_HUMAN antibody
    • Septin M antibody
    • Septin-4 antibody
    see all

Anti-SEPT4 antibody [ARTS51] (ab10440)参考文献

ab10440 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (mature human sperm)
Specification mature human sperm
Fixative 2% paraformaldehyde
Permeabilization Yes - 0.1% Trit-X on ice 15mins
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2012

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab10440 in ICC/IF, Human sperm cells. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please su...

Read More

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab10440 has never been tested in ICC/IF. All tested applications are specified on our datasheets, and these are updated as soon as any new informati...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (colon)
Specification colon
Fixative Formaldehyde
Permeabilization Yes - Triton X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"