概述

  • 产品名称Anti-SEPT12抗体
    参阅全部 SEPT12 一抗
  • 描述
    小鼠多克隆抗体to SEPT12
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-358 of Human FLJ25410

  • 阳性对照
    • Hela cells, transfected 293T cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the septin family.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SEP12_HUMAN antibody
    • SEPT12 antibody
    • Septin-12 antibody
    • Septin12 antibody

Anti-SEPT12 antibody 图像

  • Immunofluorescent analysis of FLJ25410 expression in HeLa cells using 10 µg/ml ab66960.
  • All lanes : Anti-SEPT12 antibody (ab66960) at 1/1000 dilution

    Lane 1 : FLJ25410 transfected 293T cell lysate
    Lane 2 : untransfected 293T cell lysate

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

Anti-SEPT12 antibody (ab66960)参考文献

ab66960 has not yet been referenced specifically in any publications.

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