Anti-SEPT12抗体(ab66960)

概述

  • 产品名称
    Anti-SEPT12抗体
  • 描述
    小鼠多克隆抗体to SEPT12
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-358 of Human FLJ25410

  • 阳性对照
    • Hela cells, transfected 293T cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the septin family.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SEP12_HUMAN antibody
    • SEPT12 antibody
    • Septin-12 antibody
    • Septin12 antibody

图片

  • Immunofluorescent analysis of FLJ25410 expression in HeLa cells using 10 µg/ml ab66960.

文献

ab66960 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse sperm cell)
Permeabilization
Yes - TX-100
Specification
Mouse sperm cell
Blocking step
Serum + BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Grigor Varuzhanyan

Verified customer

提交于 Mar 12 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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