重组
RabMAb

Anti-SEPP1抗体[EPR4614] (ab109514)

概述

  • 产品名称
    Anti-SEPP1抗体[EPR4614]
    参阅全部 SEPP1 一抗
  • 描述
    兔单克隆抗体[EPR4614] to SEPP1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human SEPP1.

  • 阳性对照
    • Human heart, HepG2 and 293T lysates; 293T cells
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR4614
  • 同种型
    IgG

相关产品

应用

Our Abpromise guarantee covers the use of ab109514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    图片

    • All lanes : Anti-SEPP1 antibody [EPR4614] (ab109514) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : HepG2 lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Flow cytometric analysis of permeabilized 293T cells, using ab109514 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    文献

    This product has been referenced in:
    • Sander J  et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity 47:1051-1066.e12 (2017). Human . Read more (PubMed: 29262348) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for contacting us.

    Unfortunately the BLAST came up "no significant homology" found between the immunogen and the chicken sequence.

    I hope this information is helpful to you. Please do not hesitate to contact us if ...

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    The immunogen sequence used to raise the Anti-SEPP1 antibody [EPR4614] (abx109514) is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly f...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (human colon and pancreas)
    Total protein in input
    5000 µg
    Specification
    human colon and pancreas
    Immuno-precipitation step
    Protein G
    Username

    Mr. Qing Wang

    Verified customer

    提交于 Aug 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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