功能Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
序列相似性Belongs to the peptidase C50 family.
翻译后修饰Autocleaves. This function, which is not essential for its protease activity, is unknown. Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Paraffin-embedded human colon carcinoma tissue stained for Separase using ab52210 (1/100). The right hand panel represents a negative control where ab52210 was pre-incubated with the immunising (blocking) peptide.
Western blot - Separase (phospho S801) antibody (ab52210)
All lanes : Anti-Separase (phospho S801) antibody (ab52210) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated
with EGF (200ng/ml, 30min). For this panel, ab52210 was pre-incubates with the immunizing (blocking) peptide. Lane 2 : Extracts from 293 cells treated
with EGF (200ng/ml, 30min)