Anti-Separase (phospho S1501)抗体(ab60027)

概述

  • 产品名称Anti-Separase (phospho S1501)抗体
    参阅全部 Separase 一抗
  • 描述
    兔多克隆抗体to Separase (phospho S1501)
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding S1501 of human Separase conjugated to KLH

  • 阳性对照
    • Human hepatocarcinoma tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明First purified by protein G affinity chromatography, then the antibody fraction was peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • 序列相似性Belongs to the peptidase C50 family.
  • 翻译后修饰Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Caspase like protein ESPL1 antibody
    • Caspase-like protein ESPL1 antibody
    • ESP 1 antibody
    • ESP1 antibody
    • ESPL 1 antibody
    • Espl1 antibody
    • ESPL1_HUMAN antibody
    • Extra spindle poles like 1 antibody
    • Extra spindle poles like 1 protein antibody
    • Extra spindle poles-like 1 protein antibody
    • FLJ46492 antibody
    • KIAA0165 antibody
    • Separase antibody
    • Separin antibody
    • Similar to fission yeast cut1and gene antibody
    • SSE antibody
    see all

Anti-Separase (phospho S1501) antibody 图像

  • ab60027 at 1/50 dilution staining formalin fixed, paraffin embedded human hepatocarcinoma tissue. Peroxidase conjugated secondary antibody followed by AEC staining.

Anti-Separase (phospho S1501) antibody (ab60027)参考文献

ab60027 has not yet been referenced specifically in any publications.

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