Anti-Separase抗体(ab52158)
Key features and details
- Rabbit polyclonal to Separase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Separase抗体
参阅全部 Separase 一抗 -
描述
兔多克隆抗体to Separase -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).
-
阳性对照
- human colon carcinoma, extracts from A431 cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52158于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa).
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa). |
靶标
-
功能
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. -
序列相似性
Belongs to the peptidase C50 family. -
翻译后修饰
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 9700 Human
- Entrez Gene: 105988 Mouse
- Omim: 604143 Human
- SwissProt: Q14674 Human
- SwissProt: P60330 Mouse
- Unigene: 153479 Human
- Unigene: 288324 Mouse
-
别名
- Caspase like protein ESPL1 antibody
- Caspase-like protein ESPL1 antibody
- ESP 1 antibody
see all
图片
-
All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution
Lane 1 : extracts from A431 cells
Lane 2 : extracts from A431 cells, with immunizing peptide
Predicted band size: 233 kDa
Observed band size: 233 kDa -
Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab52158 被引用在 1 文献中.
- Bacac M et al. Securin and separase modulate membrane traffic by affecting endosomal acidification. Traffic 12:615-26 (2011). PubMed: 21272169