概述

  • 产品名称Anti-SEP15抗体
    参阅全部 SEP15 一抗
  • 描述
    兔多克隆抗体to SEP15
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A synthetic peptide within residues

    VRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEF

    corresponding to amino acids 109-158 of Human SEP15 (NP_004252)

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab80985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-SEP15 antibody 图像

  • Anti-SEP15 antibody (ab80985) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

Anti-SEP15 antibody (ab80985)参考文献

This product has been referenced in:
  • Miniard AC  et al. Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression. Nucleic Acids Res 38:4807-20 (2010). Read more (PubMed: 20385601) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (primary B cells, 3T3 cells)
Loading amount 40 µg
Specification primary B cells, 3T3 cells
Gel Running Conditions Reduced Denaturing (Gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Feb 10 2012

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We have not validated this antibody for use on mouse, and therefore do not batch test it on mouse samples. If you would like to test the recent batch on a positive control from human samples, we would be happy to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"