Anti-SENP7抗体(ab58422)

概述

  • 产品名称Anti-SENP7抗体
    参阅全部 SENP7 一抗
  • 描述
    兔多克隆抗体to SENP7
  • 特异性ab58422 detects endogenous levels of total SENP7 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human SENP7.

  • 阳性对照
    • HuvEc cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab58422 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
ELISA 1/40000.

靶标

  • 功能Protease that deconjugates SUMO2 and SUMO3 from targeted proteins, but not SUMO1. Catalyzes the deconjugation of poly-SUMO2 and poly-SUMO3 chains. Has very low efficiency in processing full-length SUMO proteins to their mature forms.
  • 序列相似性Belongs to the peptidase C48 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1707 antibody
    • Senp7 antibody
    • SENP7_HUMAN antibody
    • Sentrin specific protease antibody
    • Sentrin-specific protease 7 antibody
    • Sentrin/SUMO-specific protease SENP7 antibody
    • SSP2 antibody
    • SUMO 1 specific protease 2 antibody
    • SUMO specific protease antibody
    • SUMO-1-specific protease 2 antibody
    • SUMO1/sentrin specific peptidase 7 antibody
    • SUSP2 antibody
    see all

Anti-SENP7 antibody 图像

  • All lanes : Anti-SENP7 antibody (ab58422) at 1/500 dilution

    Lane 1 : HuvEc cell extracts
    Lane 2 : HuvEc cell extracts with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 112 kDa
    Observed band size : 112 kDa

Anti-SENP7 antibody (ab58422)参考文献

ab58422 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 50 µg
Specification HEK293
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Jul 06 2016

Thank you for your enquiry. Please find the blocking protocols as below. Blocking 1. Block membrane by incubating 1 hour at room temperature or overnight at 4℃ with shaking in Blocking Solution (5% BSA, 0.05% Tween-20 in TBS (50mM Tris, 100m...

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