Anti-SENP6抗体(ab77619)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SENP6
  • 特异性
    ab77619 is expected to recognise both reported isoforms (NP_001093879.1; NP_056386.2).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-KPKYEPNPHYHEN

    , corresponding to internal sequence amino acids 778-790 of Human SENP6 (NP_056386.2).

  • 阳性对照
    • HeLa cell nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab77619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison.
    • 组织特异性
      Highly expressed in reproductive organs, such as testis, ovary and prostate.
    • 通路
      Protein modification; protein sumoylation.
    • 序列相似性
      Belongs to the peptidase C48 family.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2810017C20Rik antibody
      • FLJ11355 antibody
      • FLJ11887 antibody
      • KIAA0389 antibody
      • KIAA0797 antibody
      • SENP 6 antibody
      • Senp6 antibody
      • SENP6_HUMAN antibody
      • Sentrin specific protease 6 antibody
      • Sentrin-specific protease 6 antibody
      • Sentrin/SUMO specific protease SENP6 antibody
      • Sentrin/SUMO-specific protease SENP6 antibody
      • SSP 1 antibody
      • SSP1 antibody
      • SUMO 1 specific protease 1 antibody
      • SUMO 1 specific protease antibody
      • SUMO-1-specific protease 1 antibody
      • SUMO1 specific protease 1 antibody
      • SUMO1 specific protease antibody
      • SUMO1/sentrin specific peptidase 6 antibody
      • SUSP 1 antibody
      • SUSP1 antibody
      see all

    Anti-SENP6 antibody 图像

    实验方案

    Anti-SENP6 antibody (ab77619)参考文献

    ab77619 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (E.coli produced recombinant SENP6)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    0.2 µg
    Specification
    E.coli produced recombinant SENP6
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Apr 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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