重组Anti-SENP2抗体[EPR4356(2)] (ab124724)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4356(2)] to SENP2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SENP2抗体[EPR4356(2)]
参阅全部 SENP2 一抗 -
描述
兔单克隆抗体[EPR4356(2)] to SENP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human SENP2 aa 350-450. The exact sequence is proprietary.
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阳性对照
- MDA-MB-435, NIH:OVCAR-3, and 293T cell lysates; Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4356(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124724于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. -
序列相似性
Belongs to the peptidase C48 family. -
结构域
The N-terminus is necessary and sufficient for nuclear envelope targeting. -
翻译后修饰
Polyubiquitinated; which leads to proteasomal degradation. -
细胞定位
Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 59343 Human
- Omim: 608261 Human
- SwissProt: Q9HC62 Human
- Unigene: 401388 Human
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别名
- Axam2 antibody
- DKFZp762A2316 antibody
- KIAA1331 antibody
see all
图片
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All lanes : Anti-SENP2 antibody [EPR4356(2)] (ab124724) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-435S (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : NIH: OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 40 secondsBlocking and diluting buffer: 5% NFDM/TBST
We are not sure how to define the extra band below band of interest.
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Anti-SENP2 antibody [EPR4356(2)] (ab124724) at 1/10000 dilution + His-tagged human SENP2 recombinant protein (aa139-523)
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer: 5% NFDM/TBST
Lysate/protein at 0.01 µg per lane.
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ab124724, at 1/500 dilution, staining SENP2 in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124724 被引用在 3 文献中.
- Lund J et al. SENP2 is vital for optimal insulin signaling and insulin-stimulated glycogen synthesis in human skeletal muscle cells. Curr Res Pharmacol Drug Discov 2:100061 (2021). PubMed: 34909683
- Liu K et al. A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3. Autophagy 16:975-990 (2020). PubMed: 31373534
- Madu IG & Chen Y Assays for investigating deSUMOylation enzymes. Curr Protoc Mol Biol Chapter 10:Unit10.30 (2012). PubMed: 22870856