概述

  • 产品名称Senescence Detection试剂盒
  • 测试
    250 x 1 assay
  • 样品类型
    Tissue, Adherent cells
  • 检测类型Enzyme activity
  • 产品概述

    Senescence Detection Kit (ab65351) is designed to histochemically detect SA-beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of beta-galactosidase (SA-beta-Gal) activity, and altered patterns of gene expression.

  • 经测试应用适用于: Functional Studies, IHC-Frmore details

性能

  • 相关性Cellular senescence is a growth-arrest program by which normal diploid cells lose the ability to divide, and it plays a critical role in regulating lifespan both in vivo and in vitro. Cellular senescence occurs as reflection of organism aging and in response to internal and external stress signals.

应用

Our Abpromise guarantee covers the use of ab65351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent concentration.

Senescence Detection Kit 图像

  • Senescence-associated beta-galactosidase staining at the 2nd(A), 4th(B), 8th(C), 12th passage in vitro expansion. Cells were plated at a density of 10,000 cells/cm2 for 24h before staining. Five representative images (100x) were taken from diverse areas of cell culture, using phase-contrast microscopy to assess the number of positive cells.

    Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8:18

实验方案

Senescence Detection Kit (ab65351)参考文献

This product has been referenced in:
  • Kaur E  et al. Radiation-induced homotypic cell fusions of innately resistant glioblastoma cells mediate their sustained survival and recurrence. Carcinogenesis 36:685-95 (2015). Read more (PubMed: 25863126) »
  • Toko H  et al. Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells. J Biol Chem 289:5348-56 (2014). IHC . Read more (PubMed: 24375406) »

See all 11 Publications for this product

Product Wall

I can confirm frozen tissue sections can be used with ab65351Senescence Detection Kit. The kit has been used for frozen skin sections successfully. You can either fix the frozen tissue in the supplied fixation solution or use your preferred fixation of...

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I am sorry we have not tested plastic embedded tissue sections with this kit so we may not be able to provide you required details. You can however test this kit by keeping frozen sections as positive control.

Laminin should not interfere with the staining. The stain will bind only to SA-β-Gal. Also, you will have a normal control cell sample, which you would also grow in laminin and perform the same staining. Any blue color development observed in thes...

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ab65351 (Senescence Detection Kit) wurde nicht mit Suspensionszellen getestet oder dafür optimiert.

Da dieses Kit allerdings benutzt wird um fixierte Zellen anzufärben, könnte das folgende Protokoll vielleicht benutzt...

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Thank you for your e-mail. I am sorry about this accident.

This is to confirm that I have been liaising with the Lab to get a new vial of the 100X Staining Supplement. We need to create a datasheet and get the product from the Lab. As soon as...

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Thank you for contacting us.



Our development scientist has optimized this kit to work best at the conditions mentioned in our datasheet. But sometimes, you have to treat expts case by case and it is possible that in your case, the b...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your email.

Could you provide the purchase order number? I will sending you a replacement in your next shipment as XXXX agreed.

Thanks!

Thank you for your email. I am sorry to hear that you are experiencing problems with lot GR79964-1.

We recommend warming the staining supplement as it is in 100X concentration and frozen. It is not unusual this to be observed jelly like due to...

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Thank you for contacting us. Although we have never tried dehydrating our sections in order to mount them, I think it would be ok to do so, once you have confirmed the blue colour with the X-Gal staining

I hope this information is helpful to y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"