概述

  • 产品名称Anti-Semenogelin I抗体
    参阅全部 Semenogelin I 一抗
  • 描述
    兔多克隆抗体to Semenogelin I
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Chimpanzee, African Green Monkey
  • 免疫原

    A region within synthetic peptide: ANDHDQSRKS QQYDLNALHK TTKSQRHLGG SQQLLHNKQE GRDHDKSKGH, corresponding to N terminal amino acids 73-122 of Human Semenogelin I

  • 阳性对照
    • Jurkat cell lysate Human lung tissue Human kidney renal tubule

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
    Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
  • 组织特异性Seminal vesicle.
  • 序列相似性Belongs to the semenogelin family.
  • 翻译后修饰Transglutaminase substrate.
    Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-Inhibin-31 antibody
    • Alpha-Inhibin-92 antibody
    • Cancer/testis antigen 103 antibody
    • CT103 antibody
    • dJ172H20.2 (semenogelin I) antibody
    • dJ172H20.2 antibody
    • MGC14719 antibody
    • Semen coagulating protein antibody
    • Semenogelin antibody
    • Semenogelin I antibody
    • SEMG antibody
    • SEMG1 antibody
    • SEMG1_HUMAN antibody
    • Seminal basic protein antibody
    • seminal vesicle secretory protein 5 antibody
    • SgI antibody
    • Svp-1 antibody
    • Svp5 antibody
    • SVPIIA antibody
    • Svs2 antibody
    • SVS2P antibody
    • Svs2p2 antibody
    • Svs5 antibody
    see all

Anti-Semenogelin I antibody 图像

  • All lanes : Anti-Semenogelin I antibody (ab58692) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Semenogelin I at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Semenogelin I with ab58692 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Semenogelin I antibody (ab58692)参考文献

This product has been referenced in:
  • Teirilä L  et al. Proteomic changes of alveolar lining fluid in illnesses associated with exposure to inhaled non-infectious microbial particles. PLoS One 9:e102624 (2014). WB ; Human . Read more (PubMed: 25033447) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

It may indeed be easier to try this experiment with the rabbit monoclonal antibody ab139405. However, the immunogen information is proprietary information. In order to try to help you further could you explain what y...

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Thank you for contacting us and your interest in our products.

This is a very unusual question and I will try my best to help. The anti-Semenogelin I antibody ab58692 has been raised against a synthetic peptide taken from within the region yo...

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