Anti-Semaphorin 3F抗体(ab39956)

概述

  • 产品名称Anti-Semaphorin 3F抗体
    参阅全部 Semaphorin 3F 一抗
  • 描述
    兔多克隆抗体to Semaphorin 3F
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Semaphorin 3F.

    (Peptide available as ab39955.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Brain (Rat); Brain (Mouse); Brain (Human)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab39956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 88,120 kDa (predicted molecular weight: 88 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May play a role in cell motility and cell adhesion.
    • 组织特异性Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
    • 序列相似性Belongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Sema domain.
    • 发展阶段Detected as early as embryonic day 10.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • SEM3F_HUMAN antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted 3F antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F antibody
      • Sema III/F antibody
      • Sema IV antibody
      • SEMA-IV antibody
      • SEMA3F antibody
      • SEMA4 antibody
      • SemaIII/F antibody
      • SemaIV antibody
      • SEMAK antibody
      • Semaphorin IV antibody
      • Semaphorin-3F antibody
      • Semaphorin3F antibody
      • SemaphorinIV antibody
      see all

    Anti-Semaphorin 3F antibody 图像

    • All lanes : Anti-Semaphorin 3F antibody (ab39956) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 88 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,37 kDa,45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    Anti-Semaphorin 3F antibody (ab39956)参考文献

    ab39956 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
    Sample Mouse Cell (Brain sections)
    Specification Brain sections
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 24 2014

    Application Western blot
    Loading amount 200000 cells
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Sample Mouse Cell lysate - whole cell (Brain tissue)
    Specification Brain tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 13 2014

    Thank you for your reply. My colleague you were in correspondence with is on holiday at the moment. I checked again for you and I have to confirm that ab39955 (Semaphorin 3F peptide) is only a short peptide of 18 amino acids (used a immunogen to genera...

    Read More

    Thank you for contacting us. As you mention, we do have a number of related products available. The SEMA3F products we have in our catalog (such as ab39956) is a rabbit-polyclonal antibody against Semaphorin 3F. It is guaranteed to work with mo...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Oct 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"