概述

  • 产品名称Anti-Semaphorin 3E抗体
    参阅全部 Semaphorin 3E 一抗
  • 描述
    山羊多克隆抗体to Semaphorin 3E
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    NGGRYGTTKDYPDD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 387-400 of Human Semaphorin 3E (NP_036563.1).

  • 阳性对照
    • Human Prostate tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab112886 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab112886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.03 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 89 kDa). Preliminary experiments gave an approx 35kDa band in Human Prostate lysates. The 35kDa band was successfully blocked by incubation with the immunizing peptide.

靶标

  • 序列相似性Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Sema domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coll 5 antibody
    • Coll5 antibody
    • KIAA0331 antibody
    • M sema H antibody
    • M SEMAH antibody
    • M SemaK antibody
    • Msema H antibody
    • MSEMAH antibody
    • MSemaK antibody
    • OTTHUMP00000206419 antibody
    • OTTHUMP00000206420 antibody
    • SEM3E_HUMAN antibody
    • Sema domain immunoglobulin domain (Ig) antibody
    • Sema domain immunoglobulin domain (Ig) short basic domain secreted 3E antibody
    • Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody
    • SEMA3E antibody
    • SEMAH antibody
    • Semaphorin 3E antibody
    • Semaphorin H, mouse, homolog of antibody
    • Semaphorin-3E antibody
    • Semaphorin3E antibody
    • Short basic domain secreted (semaphorin) 3E antibody
    see all

Anti-Semaphorin 3E antibody 图像

  • ab112886 at 4µg/ml showing membranous staining of Semaphorin 3E in the secretory cells of Paraffin-embedded Human Prostate tissue by Immunohistochemistry. Detection utilised HRP staining.

Anti-Semaphorin 3E antibody (ab112886)参考文献

This product has been referenced in:
  • Mazzotta C  et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther 17:221 (2015). IHC-P ; Human . Read more (PubMed: 26292963) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E10.5 mouse embryo. Coronal section through pharyn)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citric Acid
Permeabilization Yes - 0.05% Tween 20
Specification E10.5 mouse embryo. Coronal section through pharyn
Blocking step 10% donkey serum in PBS+ 0.05% Tween20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Sophie Astrof

Verified customer

提交于 Jun 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"