The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.03 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 89 kDa). Preliminary experiments gave an approx 35kDa band in Human Prostate lysates. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
序列相似性Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Sema domain.
Sema domain immunoglobulin domain (Ig) short basic domain secreted 3E antibody
Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody
Semaphorin 3E antibody
Semaphorin H, mouse, homolog of antibody
Short basic domain secreted (semaphorin) 3E antibody
Anti-Semaphorin 3E antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3E antibody (ab112886)
ab112886 at 4µg/ml showing membranous staining of Semaphorin 3E in the secretory cells of Paraffin-embedded Human Prostate tissue by Immunohistochemistry. Detection utilised HRP staining.
Anti-Semaphorin 3E antibody (ab112886)参考文献
This product has been referenced in:
Mazzotta C et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther17:221 (2015).
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