概述

  • 产品名称Anti-Semaphorin 3B抗体
    参阅全部 Semaphorin 3B 一抗
  • 描述
    兔多克隆抗体to Semaphorin 3B
  • 特异性This antibody is specific for SEMA 3B.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Zebrafish
  • 免疫原

    A synthetic peptide made to an internal portion of the human SEMA 3B protein sequence (between residues 100-200).

  • 阳性对照
    • Mouse E16 cerebellum lysate

性能

应用

Our Abpromise guarantee covers the use of ab48197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Predicted molecular weight: 51, 83 kDa.
IHC-P 1/100.

靶标

  • 功能Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • 组织特异性Expressed abundantly but differentially in a variety of neural and nonneural tissues.
  • 序列相似性Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • 细胞定位Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SEM3B_HUMAN antibody
    • Sema A(V) antibody
    • Sema V antibody
    • Sema3b antibody
    • Semaphorin V antibody
    • Semaphorin-3B antibody
    • Semaphorin-V antibody
    see all

Anti-Semaphorin 3B antibody 图像

  • Anti-Semaphorin 3B antibody (ab48197) at 1/5000 dilution + mouse E16 cerebellum lysate
    Developed using the ECL technique

    Predicted band size : 51, 83 kDa
    Observed band size : 51,83 kDa
  • ab48197 staining Semaphorin 3B in mouse brain tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6 and then blocked in 10% serum for 1 hour at 19°C. The primary antibody was diluted in 1/100 and it was incubated in 2% blocking sera with sample for 16 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/250 as a secondary antibody.

    See Abreview

  • ab48197 staining Semaphorin 3B in human skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 10% serum for 1 hour at RT was performed. The primary antibody was used diluted 1/100 and incubated with sample in 2% serum for 15 hours at 4°C. A biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody.

    See Abreview

Anti-Semaphorin 3B antibody (ab48197)参考文献

This product has been referenced in:
  • Nguyen H  et al. Progesterone and 1,25-Dihydroxyvitamin D3 Inhibit Endometrial Cancer Cell Growth by Upregulating Semaphorin 3B and Semaphorin 3F. Mol Cancer Res 9:1479-92 (2011). WB, IHC-P ; Human . Read more (PubMed: 21933904) »
  • Esselens C  et al. The cleavage of semaphorin 3C induced by ADAMTS1 promotes cell migration. J Biol Chem 285:2463-73 (2010). Read more (PubMed: 19915008) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

提交于 Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
Username

Ms. Lucy Shaw

Verified customer

提交于 Jun 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"